Microscopy
Long-Term Live Cell Imaging![](fileadmin/_processed_/8/9/csm_incucyte_kurven_3676b040d5.png)
Sartorius Incucyte S3
The Incucyte S3 is an automated microscope located within a standard cell culture incubator and is of particular interest for low resolution, long-term, live cell imaging. It allows the independent measurement and analysis of up to 6 plates or experiments at the same time. It is the method of choice for long-term observation of spheroid and organoid development. Available objectives include 4x, 10x and 20x. These objectives are compatible with most standard cell culture plate formats and plates of to 96- or 384-wells are also supported. For the list of currently supported substrates please see (link for download). Channels available for imaging and analysis are standard brightfield/phase contrast, as well as two channels for fluorescence. Details about fluorescence can be seen below:
Color | Excitation | Emission | Possible Fluorochromes |
Green fluorescence | 440-480 nm | 504-544 nm | GFP, YFP, CellTracker Green, CFSE, FITC |
Red fluorescence | 565-605 nm | 625-705 nm | mRFP, mKate2, mCherry, CellTracker Red, Alexa Fluor 594 |
Note: The red fluorescence channel is suboptimal for the detection of fluorescence proteins such as td tomato and turbo RFP.
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Plates and Flasks fitting into IncuCyte
The list for download contains the vessels which work with the IncuCyte. Note: Only Microwell Plate formats or chamber slides will fit into our system. No flasks.