Flow Cytometry
FACS AnalysisBooking of Flow Cytometers
- Please be sure that you have attended the appropriate introductory course.
- Please write your name, phone number and your laboratory name in the booking calendar system.
- Please reserve your appointments at least two days before use.
- The maximum length of a single appointment is two hours between the hours of 11:00 and 16:00. Longer appointments should be scheduled outside of this time period.
- The appointments are binding.
- If you have to cancel an appointment, please delete it from the calendar.
- Please inform the next user when you are delayed.
Overview of Lighthouse FACS Analyzers
For FACS analysis we have a number of machines with different lasers and possible color combinations.
Overview of Lighthouse FACS Analyzers
| 355nm | 405nm | 488nm | 561nm | 638/640nm | |
|---|---|---|---|---|---|
| Beckman Coulter Gallios 1 | - | x (2) | x (5) | - | x (3) |
| Sony SP6800 Full Spectral Analyzer | - | (x 3) | (x 3) | - | (x 3) |
| Sony ID7000 Full Spectral Analyzer | (x 4) | (x 4) | (x 4) | (x 4) | (x 4) |
| BD FACS Canto | - | x (2) | x (4) | - | x (2) |
| BD Fortessa - Andy | - | x (6) | x (5) | - | x (3) |
| BD Fortessa - Frida 2 | - | x (6) | x (5) | - | x (3) |
| BD Fortessa - Vincent 2 | - | x (6) | x (2) | x (5) | x (3) |
| BD Fortessa - Leonardo | - | x (7) | x (2) | x (5) | x (3) |
| BD Fortessa - Dali | x (2) | x (6) | x (5) | - | x (3) |
() - Number of possible fluorescent detectors/Laser
1 - with carousel
2 - with HTS (compatible plates see below)
| Plate Type | Working Volume (µl) | Falcon Order No. e.g. |
| 384 flat bottom | 100 | 353961 |
| 96 well flat bottom | 275 | 351172 |
| 96 well U-bottom | 250 | 353910 |
| 96 well V-bottom | 250 | 353263 |
3 - The Sony SP6800 ZE spectral analyzer has a novel spectral detector, allowing the unmixing (separation) and analysis of multiple colors simultaneously (up to 20).
4 - The Sony ID7000 spectral analyzer has 5 decks of spectral detectors, allowing the unmixing (separation) and analysis of multiple colors simultaneously (more than 40).
Please contact the Lighthouse staff for advice on which machine would be best suited for your experiments, or for help with panel design, as well as to ask about the date and time of the next training session. For additional help with panel design, the configurations of Lighthouse analysers have been listed on the Fluorofinder website. To access them follow the links below:
See Configurations at "FluoroFinder"
Lasers & Channels of Our Flow Cytometers For Download
Fortessa Andy Lasers and Channels
Overview about lasers and suitable fluorochromes of FACS analyzer ANDY.
Fortessa Frida Lasers and Channels
Overview about lasers and suitable fluorochromes of FACS analyzer FRIDA.
Fortessa Leonardo Lasers and Channels
Overview about lasers and suitable fluorochromes of FACS analyzer LEONARDO.
Fortessa Vincent Lasers and Channels
Overview about lasers and suitable fluorochromes of FACS analyzer VINCENT.
Fortessa Dali Lasers and Channels
Overview about lasers and suitable fluorochromes of FACS analyzer DALI.
BC Gallios Lasers and Channels
Overview about lasers and suitable fluorochromes of FACS analyzer GALLIOS
BD FACSCanto Lasers and Channels
Overview about lasers and suitable fluorochromes of FACS analyzer CANTO.
If you need more information as to which combinations of dyes or fluorescent proteins it is possible to use, please contact us. The best time to do this is when first planning a new experiment.
Offline Analysis
Lighthouse also has offline analysis workstations with several flow cytometry analysis software programs that you are welcome to use:
- FlowJo
- FACSDiva
- Kaluza
- Summit
- ModFit
- FACSArray
Cell Sorting
For information about cell sorting please see our Cell Sorting Page