Dr. Josef Madl
Head of Microscopy Facility | Senior Scientist (4D Imaging)Research Interests:
- Studying functional aspects in cardiovascular systems using fluorescence lifetime imaging (FLIM) and ratiometric imaging (membrane voltage, calcium signalling)
- Determining the topology of cell arrangements in cardiac tissues via multiphoton microscopy
- Applying deconvolution-based superresolution microscopy to analyse nano- and microscale structural features in cardiac cells
Brief CV:
Josef Madl studied Biophysics at the Johannes Kepler University of Linz (graduation 2006). He continued his post-graduate studies in the laboratory of Prof. Gerhard J. Schütz, where his work focused on the analysis of store-operated calcium channels using single molecule fluorescence microscopy. After receiving his PhD in 2011, he moved to the lab of Prof. Winfried Römer, Faculty of Biology and BIOSS, University of Freiburg, where he studied the interactions of bacterial lectins with biological and synthetic membranes using different microscopy methods (confocal microscopy, widefield/TIRF- and super-resolution microscopy, atomic force microscopy). Since 2018, Josef heads the microscopy facility at IEKM and joined the 4D imaging group as a senior scientist.
Selected Publications:
- Villringer S, Madl J*, Sych T, Manner C, Imberty A, Römer W*. Lectin-mediated protocell crosslinking to mimic cell-cell junctions and adhesion. Sci Rep 2018/8:1932 *co-corresponding authors
- Stuhr-Hansen N*, Madl J*, Villringer S, Aili U, Romer W, Blixt O. Synthesis of Cholesterol-Substituted Glycopeptides for Tailor-Made Glycocalyxification of Artificial Membrane Systems. Chembiochem 2016/17:1403-1406 *co-first authors
- Mittapalli VR, Madl J, Loffek S, Kiritsi D, Kern JS, Romer W, Nystrom A, Bruckner-Tuderman L. Injury-Driven Stiffening of the Dermis Expedites Skin Carcinoma Progression. Cancer Res 2016/76:940-951
- Madl J, Weghuber J, Fritsch R, Derler I, Fahrner M, Frischauf I, Lackner B, Romanin C, Schutz GJ. Resting State Orai1 Diffuses as Homotetramer in the Plasma Membrane of Live Mammalian Cells. J Biol Chem 2010/285:41135-41142
- Muik M, Frischauf I [...] Fritsch R, Kahr H, Madl J, Gruber H, Groschner K, Romanin C. Dynamic coupling of the putative coiled-coil domain of ORAI1 with STIM1 mediates ORAI1 channel activation. J Biol Chem 2008/283:8014-8022
- Madl J, Rhode S, Stangl H, Stockinger H, Hinterdorfer P, Schutz GJ, Kada G. A combined optical and atomic force microscope for live cell investigations. Ultramicroscopy 2006/106:645-651